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A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
  • Author : Jianghui Hou
  • Publsiher : Academic Press
  • Release : 24 April 2020
  • ISBN : 0128226439
  • Pages : 420 pages
  • Rating : 4/5 from 21 ratings
GET THIS BOOKA Laboratory Guide to the Tight Junction

Summary:
A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels


A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release : 24 April 2020
GET THIS BOOKA Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches




A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo
  • Author : David K. Gardner,Michelle Lane,Andrew J. Watson
  • Publisher : Oxford University Press
  • Release : 08 January 2004
GET THIS BOOKA Laboratory Guide to the Mammalian Embryo

Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.



The Paracellular Channel

The Paracellular Channel
  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release : 08 June 2018
GET THIS BOOKThe Paracellular Channel

The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure






Skin Barrier Function

Skin Barrier Function
  • Author : T. Agner
  • Publisher : Karger Medical and Scientific Publishers
  • Release : 04 February 2016
GET THIS BOOKSkin Barrier Function

Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary




The Impact of Food Bioactives on Health

The Impact of Food Bioactives on Health
  • Author : Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers
  • Publisher : Springer
  • Release : 29 April 2015
GET THIS BOOKThe Impact of Food Bioactives on Health

“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify